Products / works at µm
Photo-patterning with PRIMO
Alvéole builds bioengineering tools that make cell models more reliable and reproducible by controlling the cell microenvironment. PRIMO does micropatterning, hydrogel polymerization and microfabrication in a single device, so you can create bespoke in vitro microenvironments for cell biology and cryo-ET studies.
Supplied in the U.S. by nanoscaleLABS
How PRIMO works
Contactless and maskless
A digital micromirror device shapes UV light into the pattern you load, so no mask or physical contact is needed. Load any grayscale pdf, tif or png image into the LEONARDO software.
Quantitative
Tuning the UV dose controls the protein density of the pattern.
Any surface
Pattern flat, curved or microstructured surfaces, including microwell walls and PDMS micropillars, on standard substrates such as PDMS, glass, plastic, NOA and SU8.
High throughput
Pattern 96-well plates. The PLPP Gel reagent speeds up micropatterning experiments by up to 30 times.
New bioprinter
PRIMO 405: bioprinting for 3D cell culture
PRIMO 405 is Alvéole's new bioprinter, optimized for precise cell embedding in various hydrogels. Its 405 nm, cell-friendly wavelength preserves viability while giving you full creative control over micro-environments. It is aimed at tissue engineering, for organ and disease modeling.
Print detailed patterns and precise structures in various hydrogels, with or without embedded cells. The PRIMO Optical Platform is a ready-to-use option with pre-integrated optics, so you do not need a dedicated microscope.
The PRIMO product line
- PRIMO 2 The new-generation module. Compact footprint, a new optical design that allows epi-fluorescence microscopy, and faster performance. Micropatterning, hydrogel polymerization and microfabrication in a single device.
- PRIMO 405 A bioprinter for 3D cell culture. Embeds cells precisely in various hydrogels using cell-friendly 405 nm light, with direct in-well printing up to 384-well plates and 5 µm resolution.
- PRIMO platform PRIMO 2 technology with integrated optics, so you do not need a dedicated microscope. Choose the Micropatterning platform for micropatterning only, or the Advanced platform for micropatterning, hydrogel polymerization and microfabrication.
- LEONARDO 5 The software that controls PRIMO. Built in modules for micropatterning, EM grid micropatterning, multiwell plate micropatterning, hydrogel structuration, microfabrication and multi-image projection.
- PLPP Classic Photoactivatable reagent for high-throughput micropatterning of cell culture substrates under PRIMO illumination.
- PLPP Gel Photoactivatable reagent that speeds up micropatterning experiments by up to 30 times.
- HyPE Photoactivatable reagent for flexibly structuring hydrogels in 2.5D.
- PDMS stencil A multiwell stencil that multiplies experimental conditions on one substrate and reduces the volume of reagents used.
Applications
- 2D micropatterning of single cells and cell populations
- Patterning on 3D microstructures
- Microfabrication and organ-on-a-chip molds
- Hydrogel polymerization and structuration
- Multiwell plate micropatterning
- Micropatterning for cryo-ET and EM grids
- Tissue engineering
- Organ and disease modeling
Ask about micro-patterning and micro-fabrication
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nanoscaleLABS, LLC
Fairfax, VA 22030, USA
(703) 662-3276
info@nanoscalelabs.com